In this contribution we assessed the biomass production in sporocarps of macromycetes in a sub-mountainous beech stand with plots of varied stocking density during 2007 and 2008. The individual partial research plots (PRPs) had stocking degrees from 0.0 for clear cut to 0.9 for the intact control. The total number of macromycetes species identified in the stands was 162. 90 of which were suitable for production evaluation. The dynamics of biomass production in fruiting bodies during the entire study period exhibited a trend which increased from the clear cut (0.86 and 2.23 kg.ha-1) to the densest PRP (44.5 and 248.5 kg.ha-1 fresh mass of fruiting bodies). The biomass production in sporocarps was mostly due to ectomycorrhizal macromycetes, which produced a total of 327.7 kg.ha-1, and especially to the Craterellus cornucopioides, Lactarius piperatus and Russula genus species. Among lignicolous macromycetes, the highest biomass was produced by Armillaria ostoyae, Hypholoma sublateritium and Kuehneromyces mutabilis species. The lowest biomass amount of 5.3 kg.ha-1 was produced by terrestrial saprophytic macromycetes, where their fruiting was limited by unfavourable climatic and ecological conditions, especially on the partial research plots with the lowest stocking density.