Eleven lakes belonging to four freshwater systems were investigated in the Třeboň Basin Area (Cep, Tušt, Halámky and Veselí) in 2004. These lakes, some over ten meters deep were formed after completion of mining activities under the groundwater level. The majority of them are slow flow rate systems and have oligotrophic to mesotrophic character. The succession of plant and animal associations was affected by various types of post-mining activities (recreation, angling and forestry). Phytocoenological relevés of littoral stands and floristic lists of plants concluded that the sandpit lakes are suitable localities not only for plant species common in other wetland biotopes (i.e. fishpond littorals) but also for species protected by the Environmental Law - CZ No. 395/1992. The presence of endangered species of plants and animals has been recorded in a number of sandpit lakes after discontinuation the sand mining.