Changes in land cover temperatures as a result of anthropogenic intervention in the water regime in selected localities of the Šumava mountains (the Czech Republic) were identified. The research was conducted in two subcatchments of similar size, altitude and relief. The subcatchment of the Bonarův stream was chosen as an area affected by water drainage. Subcatchment of the Ferdinandův stream, not affected by drainage, was chosen as a reference area. Distribution of land cover temperatures measured directly and obtained from Landsat TM satellite data was evaluated for both catchments. Temperature recording was performed in four locations of each of the catchments and in four levels of spruce forest between July 7 and November 4 of 2005. Comparison of the temperature values of both subcatchments showed that the temperature behaviour in both areas did not differ in average but was significantly different in its variance and mainly in daily amplitudes. Results show that in the drained area, the temperatures fluctuate more during the day. The processed satellite data show that the differences in temperature values between the subcatchments are significant and that the drained area shows higher temperatures. Results support an application of the Landsat TM data for evaluation of the land cover temperature as pattern of landscape functioning.