The characteristics of the landscape and its individual elements (natural and socio-economic resources) represent the main factors of the regional development. Localisation of natural and socio-economic resources in the region determines basic potential for development of individual human activities. Usually the resources and potentials are not utilised in a most appropriate way. The inadequate utilisation of the individual resources is the basic reason for endangering and deteriorating of these resources, their qualitative and quantitative properties. The paper presents an example of the classification of regions of the Slovak Republic on the base of their environmental conditions. The basic criteria for classification were: the landscape-ecological significance of the regional resources on one side and load on these resources by stress factors on the other side. The landscape-ecological significance indicates the representation and their importance in the particular regions and loading by stress factors indicates environmental problems of the regions resulting from inappropriate utilisation of the individual region resources.