The work presents survey results reached in solving the problems of explant cultures of selected introduced and native woody species. Main attention is being paid to callus and organ cultures. From organ cultures regeneration possibilities of horse chestnut (Aesculus hippocatanum L.) from apical buds, of bower actinidia /Actinidia arguta Sieb. et Yucc. (Miq.) and of Yangtao actinidia (A. Chinensis Planch.) from axillary buds, of Spanish chestnut (Castanea sativa Mill.), (Thuja occidentalis L. cv. malonyana), Scotch pine (Pinus sylvestris L.), Armad pine (P. armandii Franch.) and larch (Larix deciua Mill.) from isolated embryos were studied. The influence of the donor woody plant, sampling period, and culturing medium composition on growth and development of explants was confirmed. The method of organ cultures proceeding from juvenile tissues represents a  perspective possibility how to cultivate regenerats.