The microorganisms play an important role in evolution and expansion of life on the Earth and in maintenance of individual ecosystems. Essentially, the total ecological balance of the biosphere is determined by the activity of microorganisms. The microorganisms are a source of many valuable compounds and useful information, too. For this reason it is necessary to make an effort for their isolation and characterization. Nowadays, the study of biodiversity is considered to be one of the most important research trends of the biological sciences and is also one of the main activities of the Center of Excellence for Protection and Use of Landscape and Biodiversity of the Operational Programme Research and Development financed by the European Regional Development Fund. Yeasts are also important members in many ecosystems and form significant part of the microbial population on leaves. The monitoring of yeast community has a fundamental role for understanding the functioning of their relationships in various natural environments. The information about the structure and diversity of yeast community will be a part of the created databasis which will be integrated into the system enabling the access from the other partner workplaces; this will result in the integration of workplaces studying the biodiversity as an integral part of landscape in the Center of Excellence.