The character of tourism and landscape interactions is very complicated as it requires complex landscape-ecological approach and environmental limits consideration. It could be solved thrue the development and the application of new quantification methods for natural localization assumptions, what is the aim of our study, using Bratislava IV as a case study. In our approach landscape-ecological evaluation of landscape structure and appropriate tourism forms and activities taking into account the landscape diversity, genofond significance, generic rarity and biotope vulnerability is considered as the essence of ecological actvities of tourism The basis for spatial representation of ecosystems was the processing of secondary landscape structure map. The representation of landscape cover classes was processed in vector format as it was based on a synthesis of thematic and relevant layers contained in ZB GIS. Elements mapping units were proposed under the legend of Corine Land Cover on the 3rd mapping level and verified fieldwork in the 2014 year. As the attractive and important landscape segments were identified elements representing the 1st (e.g. alluvial forests, oak-hombean forests, wetlands), 2nd (e.g. bank growth) and 3rd (e.g.vineyards, recreational areas) 4th (arable land, multifunctional areas with mosaic of green areas)) and 5th (industrial zones) category of landscape-ecological significance,