The aim of the paper is to evaluate the way of providing spatial information sources based on selected parameters with a focus on the development of a web map application for the assessment of the natural capital of the landscape of Slovakia. At the same time, the possibilities of their further processing and interpretation in web map applications for the needs of the assessed area are evaluated. The results point to the fact that most of the assessed information is available at least in the form of a display in a map application window or as a web map service for further processing in a desktop GIS. However, the description of metadata and the lack of consistency of datasets in the context of the INSPIRE Directive appear to be insuffi cient. At the same time, it can be stated that most of the evaluated applications do provide basic possibilities for working with map data, but from the point of view of advanced tools for further data processing, the evaluated applications are not suffi cient for assessing the potential of the territory and do not use the full potential of geoinformation and web technologies. The output of the paper also serves as an overview of publicly available spatial information in the fi eld of ecology, landscape ecology, environmental science, as well as other related disciplines for the territory of Slovakia. This resource can be used for the preparation of scientifi c studies (e.g. analyses, syntheses, interpretations), the creation of mapping documents or the design and implementation of mapping applications with an emphasis on the assessment of the natural capital of the landscape.