Plant habitus and the creation of secondary metabolites in the chamomile plants are depended on the endogenous and exogenous factors, which can be divided in two groups): a) morph – ontogenetic variability, b) genetic variability resp. genetic determination. The activity of these factors is reflected in the biomass production, flower drug production, content and composition of essential oil and some other characteristics of chamomile populations. This study presents the genetic and environmental variation of essential oil and its sesquiterpene composition (/-/-α-Bisabololoxide A, /-/-α-Bisabololoxide B, /-/- α-Bisabolol and Chamazulene) of autochthonous growing chamomile population in comparison with the variety ‘LIANKA’, which is cultivated in the Eastern Slovakia. Based on the study of pharmacodynamics properties, the chamomile variety LIANKA' was bred at the University of Presov, Slovakia, between the years 2008 – 2013. Currently, this variety obtains the Certificate by the Community Plant Variety Office (CPVO) in Angers, France (in 2018). The GC/MS characteristics of the wild grow chamomile population on the Eastern Slovakian Lowland confirm the bisabololoxide chemotype with high content of /-/-α-bisabololoxides A and B (49.20 ± 0.80 %). The selected variety, environmental conditions, agronomic management practice and effective distillation were obtained of substantial contents of /-/-α-bisabolol (68.00 ±2.82 %) and chamazulene (10.50 ± 1.04 %) and the low contents of /-/-α bisabololoxides A and B (2.75 ± 0.32 %). The blue essential oil content was ranged from 0.60 to 0.64 % in order to time of single flower harvests. The chamomile variety 'LIANKA' and its large-scale cultivation, extraction and distillation is very forward as raw-material to the pharmaceutical industry in the world.