The structure of the mycobiota was investigated on mycological permanent research plots of different aged spruce stands planted on former agricultural land in the Veporské vrchy Hills in central Slovakia. A total of 110 ectomycorrhizal (EKM) and saprotrophic (SAP) species of macromycetes were determined, of which 106 species (96.4%) were suitable for evaluating fruiting body biomass production. The values of the mycorrhizal potential in individual stands of different types and in individual research years varied from 0.9 to 2.1, while the average values of 1.3 to 1.5 for individual stands can be considered quite favorable. The most dominant EKM species were Ramaria formosa, Hygrophorus pustulatus, Lactarius aurantiacus. The most dominant SAP species were Rhodocollybia butyracea, Ampulloclitocybe clavipes, Mycena cinerella. The most fruiting bodies (19,583 ex.) were produced by SAP species, the highest values of fresh biomass of fruiting bodies (5,049 kg.ha-1) were produced by EKM species. Our results confirm the strong mycorrhizal potential of the ectomycorrhizal mycobiota depending on the age of the stands. In general, EKM macromycetes dominate the youngest stands. In the oldest stands, SAP macromycetes dominate due to soil-humification conditions (soil acidification, thickness of raw humus, needle fall) similar to a natural spruce forest.